Partial Purification and Characterization of the mRNA for α-Amylase from Barley Aleurone Layers
Open Access
- 1 May 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 65 (5) , 834-837
- https://doi.org/10.1104/pp.65.5.834
Abstract
The poly(A)-containing mRNA from barley aleurone layers pretreated with gibberellic acid has been purified by phenol-chloroform extraction and repeated oligo[d(pT)]-cellulose chromatography. This RNA has been translated in both the wheat germ and reticulocyte lysate in vitro translation systems with greater than 50% of the synthesized protein being α-amylase. The mRNA for α-amylase has been further purified by dimethylsulfoxide-formamide-sucrose density gradient centrifugation and by gel electrophoresis. By these methods, its molecular weight has been determined to be 580,000.This publication has 14 references indexed in Scilit:
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