Glutamate oxaloacetate transaminase isozymes of the Triticinae: dissociation and recombination of subunits

1 January 1977

journal article
research article
Published by Springer Nature in Theoretical and Applied Genetics

Vol. 50 (1) , 47-51
https://doi.org/10.1007/bf00273797

Abstract

A simple procedure has been developed for the dissociation of active molecules of glutamate oxaloacetate transaminase (GOT: E.C. 2.6.1.1) into protomers and for the reassociation of the subunits into active enzymes. Results of experiments in which the protomers of genetically controlled electrophoretic variants of GOT of Triticum aestivum and of several related species were dissociated and recombined in crude tissue extracts and in partially purified preparations support the hypothesis that the enzyme exists functionally as a dimer in the Triticinae.

Keywords

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