Cytology of the Superficial Keratinised Cells in Experimental Keratitis Sicca

Abstract
Keratoconjunctivitis sicca was produced in 2 rabbits by the surgical extirpation of the tear-secreting glands and the nictitating membrane in 1 eye. Flat mounts of the superficial corneal epithelium were prepared by the in vivo replica technique. Histomorphological and cytological studies of the keratinized cells showed irregularly arranged, crumpled and piled-up cells. The cells contained coarse granular and fibrillar material in the cytoplasm and large granules in the nuclei. The reaction for -S-S- bridges was positive in these cells. They showed birefringence under polarization microscopy, but possessed some ribonucleoproteins.

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