TRANSFERRIN BINDING AND IRON TRANSPORT IN IRON-DEFICIENT AND IRON-REPLETE RAT RETICULOCYTES

  • 1 January 1979
    • journal article
    • research article
    • Vol. 93  (4) , 645-651
Abstract
Three aspects of Fe metabolism were studied in reticulocytes from Fe-deficient, phlebotomized, and phenylhydrazine-treated rats: the number of transferrin binding sites, the uptake of 59Fe-transferrin and the ability of cytosol to mobilize 59Fe from 59Fe-labeled reticulocyte plasma membrane. The number of transferrin binding sites, assayed by meauring the nding of 125I-labeled transferrin to reticulocytes, were similar in Fe-deficient and phlebotomy-induced reticulocytes, 66,000 and 75,000 binding sites/cell, respectively, but were about doubled, 120,000 binding sites/cell in phenylhydrazine-induced reticulocytes. Uptake of 59Fe into Fe-deficient reticulocytes was about 1/2 that for phlebotomy-induced reticulocytes and 1/4 that for phenylhydrazine-induced reticulocytes, the rates of uptake being measured as 21,542, 41,233 and 79,600 molecules of 59Fe/min per cell, respectively. The mobilizing activity of cytosol free Fe-deficient reticulocytes was also about 1/2 that of cytosol from phlebotomy-induced reticulocytes and about 1/4 that of cytosol from phenylhydrazine-induced reticulocytes. Instead of a compensatory increase in these aspects of Fe metabolism, the Fe-deficient reticulocyte apparently had a decreased ability to transport Fe.

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