Studies on Isolated Protoplasts and Vacuoles: I. GENERAL PROPERTIES

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Abstract
Protoplasts and vacuoles have been isolated in large quantities from the parenchymatous placental tissue of immature tomato fruit. Their mechanical stability and osmotic stability have been investigated. A small proportion of initially discrete but adjacent vacuoles were seen to coalesce; the possible significance of this phenomenon in the growth of plant cells is discussed. The overall chemical composition of isolated protoplasts has been determined, and their lipids have been analysed by thin-layer chromatography.