Observations of Nuclei in the Moving Mitochondria of Live Physarum Cells by Means of a Double Fluorescent Staining Technique, Using Ethidium Bromide and Dimethylaminostyrylmethylpyridiniumiodine (DASPMI)

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Abstract
Ethidium bromide has been shown to be useful as a probe to observe mitochondrial nuclei (mt-nuclei) in the moving mitochondria in live cells. In P. polycephalum, a double staining technique, using ethidium bromide and dimethylaminostyrylmethylpyridiniumiodine, detects both changes in mitochondrial distribution and orientation, and changes in the shape of the mt-nucleus during the mitochondrial division cycle. When dumbbell-shaped mitochondria in live cells divide to form daughter mitochondria, each mt-nucleus also divides, separating into the daughter mitochondria.