Suppression of Shear-Induced Hemolysis by Three Plasma Proteins
- 1 January 1976
- journal article
- research article
- Published by Taylor & Francis in Biomaterials, Medical Devices, and Artificial Organs
- Vol. 4 (1) , 21-48
- https://doi.org/10.3109/10731197609118643
Abstract
Hemolysis was induced in erythrocyte [human] suspensions by shearing between rotating metal disks 10 cm in diameter at 325 rev/min; stresses did not exceed 100 dynes/cm2. The role of proteins (.gamma.-globulin, G; albumin, A; fibrinogen, F) in the environment was tested in 2 ways. First, disks were precoated by exposure to protein solutions of physiologic concentration and then used to hemolyze the suspensions. Test carried to 104 s showed protective effects, relative to shearing with virgin disks, with a ranking G > A > F. Second, proteins were dissolved in the suspension media and hemolysis compared to the case without protein. Large suppression of hemolysis was found during storage (G .apprxeq. A > F) but only G offered significant protection against shear.This publication has 9 references indexed in Scilit:
- Hemolysis artifacts induced in rotating shear devicesJournal of Biomedical Materials Research, 1974
- The conformation of adsorbed blood proteins by infrared bound fraction measurementsJournal of Colloid and Interface Science, 1974
- Effect of surface materials on shear-induced hemolysisJournal of Biomedical Materials Research, 1972
- Red Blood Cell Damage by Shear StressBiophysical Journal, 1972
- Hemolysis in simple shear flowsAIChE Journal, 1970
- Initial events in interactions of blood with a foreign surfaceJournal of Biomedical Materials Research, 1969
- Microstructure of initial thrombus formation on foreign materialsJournal of Biomedical Materials Research, 1969
- Ribonuclease adsorption on glass surfacesArchives of Biochemistry and Biophysics, 1965