Production of Monoclonal and Monospecific Antibodies against Non-capsid Proteins of Poliovirus
- 1 November 1986
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 67 (11) , 2415-2422
- https://doi.org/10.1099/0022-1317-67-11-2415
Abstract
Non-capsid poliovirus proteins of the P2 region in extracts of infected cells were solubilized by SDS separated by PAGE, electroeluted from the gels and used to immunize mice. The sera obtained were rendered monospecific by extensive absorption with proteins from uninfected cell extracts, and the spleen cells of these animals were used to establish antibody-secreting hybridomas. The monoclonal and the monospecific antibodies recognized denatured antigen (proteins 2C, 2BC and P2) in ELISA, immunoblot and immunoprecipitation tests and also combined with the native proteins of the P2 region in immunoprecipitation. In addition, the antibodies can be used successfully for immunofluorescence and electron microscopic immunocytochemistry.This publication has 21 references indexed in Scilit:
- Poliovirus neutralization epitopes: analysis and localization with neutralizing monoclonal antibodiesJournal of Virology, 1982
- Antibodies against a synthetic peptide of the poliovirus replicase protein: reaction with native, virus-encoded proteins and inhibition of virus-specific polymerase activities in vitroJournal of Virology, 1982
- Proteolytic processing of poliovirus polypeptides: antibodies to polypeptide P3-7c inhibit cleavage at glutamine-glycine pairs.Proceedings of the National Academy of Sciences, 1982
- Resin development for electron microscopy and an analysis of embedding at low temperature*Journal of Microscopy, 1982
- Specimen preparation for electron microscopy using low temperature embedding resinsJournal of Microscopy, 1982
- A membrane-associated precursor to poliovirus VPg identified by immunoprecipitation with antibodies directed against a synthetic heptapeptideCell, 1982
- A simple and rapid method for the quantitative isolation of proteins from polyacrylamide gelsAnalytical Biochemistry, 1981
- Kinetics and location of poliovirus macromolecular synthesis in correlation to virus-induced cytopathologyVirology, 1980
- Isolation of a soluble and template-dependent poliovirus RNA polymerase that copies virion RNA in vitroJournal of Virology, 1979
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979