INHIBITION OF BENZO(A)PYRENE-INDUCED TRANSFORMATION OF C3H-1OT1-2 CELLS BY ALLYLISOPROPYLACETAMIDE AND ISOPROPYLVALERAMIDE

  • 1 January 1981
    • journal article
    • research article
    • Vol. 41  (5) , 1893-1897
Abstract
The influence of allylisopropylacetamide (AIA) and isopropylvaleramide (IVA) on the in vitro transforming ability of 2 potent carcinogens, benzo(a)pyrene [B(a)P] and 7,12-dimethylbenz(a)anthracene, on C3H1OT1/2 cells [mouse embryo fibroblast] was investigated. Both AIA and IVA showed a dose-dependent inhibition of B(a)P-induced transformation of C3H1OT1/2 cells when added simultaneously for 24 h with the carcinogen. While pretreatment, simultaneous treatment and postreatment of the cells with AIA or IVA inhibited transformation, the 24 h posttreatment was somewhat more effective. The protective effect did not appear to result from alterations in B(a)P metabolism inasmuch as aryl hydroxarbon hydroxylase activity and the metabolic products of B(a)P detected by high-pressure liquid chromatography were not changed by AIA or IVA treatment. AIA and IVA did not selectively kill chemically transformed C3H1OT1/2 cells, as indicated by the absence of their effect on an established, chemically transformed cell one. AIA and IVA also inhibited 7,12-dimethylbenz(a)anthracene-induced transformation of C3H1OT1/2 cells. AIA and IVA may be useful protective agents. They presumably exert their protective effect at some dosage between the activation of the carcinogen and the expression of the transformed phenotype.