Comparative analysis of nuclear proteins of B cells in different developmental stages

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Abstract
The developmental stage‐specific regulation of V(D)J recombination, a gene rearrangement process of antigen receptor gene segments, is tightly controlled in cells. Here we screened proteins uniquely or differentially expressed among three developmentally distinguishable B cells (pro‐B, pre‐B and mature B cells) using two‐dimensional gel electrophoresis and mass spectrometry. Chromatin assembly factor 1 was uniquely expressed in pro‐B cells. Purine nucleotide phosphorylase, LCK, E2A and many other unidentified proteins were dominantly present in the nucleus at the early stage of B cell development where the V(D)J recombination process occurs. Also, few proteins including guanidine nucleotide binding proteins were exclusively expressed in pre‐B cell. Such findings can provide some information to help understand the developmental regulation of gene rearrangement occurring during B cell development.