Degradation of a Polymerase Chain Reaction (PCR) Product by Heat‐Stable Deoxyribonuclease (DNase) Produced from Yersinia enterocolitica
Open Access
- 1 February 1994
- journal article
- research article
- Published by Wiley in Microbiology and Immunology
- Vol. 38 (2) , 153-156
- https://doi.org/10.1111/j.1348-0421.1994.tb01757.x
Abstract
When crude deoxyribonucleic acid (DNA) preparations by boiling were used for the polymerase chain reaction (PCR) from pathogenic and non‐pathogenic Yersinia enterocolitica strains, the amplified products were degraded after their storage at 4 C. The degradation of products was prevented by the addition of ethylenediaminetetraacetate (EDTA) or treatment with proteinase K. These findings indicate that Y. enterocolitica produced heat‐stable deoxyribonuclease (DNase). Proteinase K treatment would be recommended to prevent heat‐stable DNase contamination in the DNA preparations for PCR from Y. enterocolitica strains.Keywords
This publication has 6 references indexed in Scilit:
- PCR products generated from unpurified Salmonella DNA are degraded by thermostable nuclease activityLetters in Applied Microbiology, 1993
- Detection and identification of Yersinia pseudotuberculosis and pathogenic Yersinia enterocolitica by an improved polymerase chain reaction methodJournal of Clinical Microbiology, 1992
- [21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reactionPublished by Elsevier ,1987
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985
- Rapid procedure for detection and isolation of large and small plasmidsJournal of Bacteriology, 1981
- Characterization ofYersinia enterocolitica sensu strictoCurrent Microbiology, 1980