Simultaneous determination of retinol and retinyl esters in serum or plasma by reversed-phase high-performance liquid chromatography.
Open Access
- 1 November 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 24 (11) , 1920-1923
- https://doi.org/10.1093/clinchem/24.11.1920
Abstract
We propose a single-run liquid-chromatographic determination, with ultraviolet detection at 330 nm, for serum retinol and retinyl esters. The vitamin A derivatives are extracted according to the Bligh-Dyer procedure. With 200 microliter or serum, the lower detection limit is 50 microgram/liter for retinol and about 100 microgram/liter for retinyl esters. Within-run precision (CV) was 2.3% for retinol, 4.3% for retinyl palmitate. Day-to-day percision (CV, n = 20) for retinol was 4.9% during a month. The method can be used for the assessment of vitamin A absorption tests and for the determination of serum retinol (normal, subnormal, and above-normal concentrations). Serum retinyl esters can only be measured in conditions where concentrations exceed 100 microgram/liter.This publication has 0 references indexed in Scilit: