Proliferative Activity of Human Granulocytes, as Estimated Quantitatively by In Vitro Colony Formation

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Abstract
With methylcellulose-enriched medium and colony-stimulating (actor prepared from human-embryo kidney cells, an average of 2.4 colonies/10,000 marrow nucleated cells plated can be formed from marrows of normal subjects. Colony formation was markedly reduced in plated marrows from patients with acute leukemia in relapse and aplastic anemia. The proportion of granulocyte precursors in the marrow and the number of colonies in vitro produced were linearly related. Marrow from normal volunteers produced 6 colonies/10,000 granulocyte precursors (cells younger than metamyelocytes). As many as 126 colonies/10,000 granulocyte precursors were cultured from marrow of patients with acute leukemia recovering from chemotherapy. By use of nucleated cell count of the marrow aspirate and colony production, the number of colonies/ml could be determined. The median and range from 10 normals were 12,000 colonies/ml (2,000–25,000). The kinetics of marrow granulocyte function could be studied serially by use of calculations of colony formation, colonies/10,000 granulocyte precursors, and the nucleated cell count in patients with acute lymphocytic leukemia undergoing treatment.

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