THE PROCESS OF DIFFERENTIATION OF EMBRYOID BODIES IN THE LUNG OF SYNGENEIC MICE

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Abstract
Differentiation of embryoid bodies of mouse testicular teratocarcinoma OTT6050 transplanted into the lung of syngeneic mice (129/Sv) is described. Embryoid bodies took more than 2 wk to differentiate, and several kinds of differentiated tissues appeared often in the colonies derived from a single embryoid body. All colonies with differentiated tissues were larger than 100 .mu.m in diameter. Three steps in differentiation of embryoid bodies were distinguished by microscopic observations on histological preparations of tumors at different periods after injection. The 1st step is deformation of embryoid bodies and disappearance of the outer endodermal cells, which occurs within a few days after injection. In the 2nd step, which begins 5-7 days after injection, clusters of embryonal carcinoma cells in the colony are identified by the PAS [periodic acid schiff] reaction. The 3rd step starts about 10 days after injection and is characterized by formation of tubular structures in some clusters.