Promoter for the establishment of repressor synthesis in bacteriophage lambda.
- 1 June 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (6) , 3191-3195
- https://doi.org/10.1073/pnas.77.6.3191
Abstract
Transcription of the lambda repressor gene (cI) is positively regulated by the phage-encoded proteins cII and cIII. We have isolated and characterized the 5'-terminal region of this RNA and shown that it originates at a promoter (pE) located between genes cro and cII. The DNA sequence of this promoter shows little homology to other known promoters. Initiation of transcription from PE is abolished by the cis-dominant mutations cY; these mutations alter the "-10" and "-35" regions of the promoter. We propose that the "-35" region is the site of activation of PE, possibly via the direct interaction of protein cII.This publication has 37 references indexed in Scilit:
- Interaction site of Escherichia coli cyclic AMP receptor protein on DNA of galactose operon promoters.Proceedings of the National Academy of Sciences, 1979
- Location of the regulatory site for establishment of repression by bacteriophage lambda.Proceedings of the National Academy of Sciences, 1979
- Mutants of bacteriophage λ which do not require the cIII gene for efficient lysogenizationVirology, 1978
- The relationship between function and DNA sequence in an intercistronic regulatory region in phage λNature, 1978
- Nucleotide sequence of cro, cII and part of the O gene in phage λ DNANature, 1978
- Sequence of cro gene of bacteriophage lambdaNature, 1977
- RNA polymerase binding sites in λp lac 5 DNAProceedings of the National Academy of Sciences, 1977
- A ribosome binding site from the PR RNA of bacteriophage lambdaJournal of Molecular Biology, 1977
- Rat Insulin Genes: Construction of Plasmids Containing the Coding SequencesScience, 1977
- The 5′ terminal nucleotide of RNA from vesicular stomatitis virus defective interfering particlesVirology, 1977