Purification and chemical characterization of α‐fetoprotein from rat and mouse

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Abstract
Alpha‐fetoprotein of the rat was immunochemically purified and characterized. Mouse α‐fetoprotein, which cross‐reacts with antibodies to rat α‐fetoprotein, was also purified. Rat α‐fetoprotein was heterogeneous in disc electrophoresis and isoelectric focusing and had two different isoelectric points at pH 4.76 and 5.05. Mouse α‐fetoprotein was homogeneous when analyzed similarly. The molecular weight of both rat and mouse α‐fetoprotein was 70,000 as determined by gel electrophoresis in the presence of sodium dodecyl sulfate. The two specimens revealed a close similarity also in some other physicochemical, chemical and immunological properties.