RADIATION-INDUCED TRANSFORMATION OF 10T1/2 MOUSE CELLS IN THE PLATEAU PHASE - POST-IRRADIATION CHANGES AND SERUM DEPENDENCE

  • 1 January 1981
    • journal article
    • research article
    • Vol. 72  (5) , 762-768
Abstract
The frequency of transformation induced in plateau phase 10T 1/2 [mouse embryo fibroblast] cells by a single dose of X-rays decreased as a function of time during the early post-irradiation period (0-6 h) and tended to increase during the late post-irradiation period (7-48 h). The extent of reduction as measured in terms of the ratio (6 h/0 h value) of transformation ranged from 0.2-1.1, depending upon the batch of serum which was used for pre-irradiation culture. The above findings, together with the result of a serum-free experiment, suggested that a factor affecting such reduction is present in sera. Transformation frequency depended not only on the serum used for pre-irradiation culture but also on the serum for assay culture. To maintain internal consistency of results from a series of experiments, the use of a single batch of serum is essential.

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