Callus formation and induction of a cell suspension culture from Acacia senegal
- 1 October 1986
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 5 (5) , 365-367
- https://doi.org/10.1007/bf00268603
Abstract
Cell proliferation has been induced from the cambial zone of a branch of Acacia senegal, kept on the basal Knop and Ball medium in the presence of auxin. Transferred on the more complete nutrient medium of Schenk and Hildebrandt, the colonies gave rise to several cell lines. One of the friable lines, consisting of aggregates of parenchymatous cells, gave a cell suspension culture in an agitated liquid medium which is maintained as a strain of illimited growth. The heterogeneous suspension did not undergo noticeable changes after eight transfers. Aggregates collected on a 1000-μm nylon seive were able to grow on a solid medium and gave back a friable callus similar to the initial colonies.Keywords
This publication has 3 references indexed in Scilit:
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- Acacia koa Gray plantlets from somatic callus tissueJournal of Heredity, 1976
- Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell culturesCanadian Journal of Botany, 1972