A simple preservative for flow cytometric DNA analysis
Open Access
- 1 January 1989
- Vol. 10 (1) , 86-89
- https://doi.org/10.1002/cyto.990100115
Abstract
A solution containing citric acid buffered saline (CABS) and 99% ethanol (E) 1:1 was used for preserving cells for flow cytometric DNA analysis. DNA histograms obtained from fine needle biopsy aspirates and preserved in CABS+E had a similar mean coefficient of variation (CV) as was obtained from aspirates taken in CABS (3.3 vs. 3.4%) and a clearly smaller mean CV than was obtained from aspirates preserved in 50% ethanol (mean 4.8%, P < .0001). Aspirates taken in CABS more often contained a small (< 3,000) number of cells as compared with aspirates preserved either in CABS+E or ethanol (P < .0001). Since preservation of cells in CABS+E allows long‐term storage of samples and results in a decreased number of insufficient samples as compared with buffered saline and in an enhanced resolution as compared with 50% ethanol, CABS+E is recommended for preservation of cytological samples to be analyzed for DNA content with flow cytometry.Keywords
This publication has 13 references indexed in Scilit:
- Comparison of fresh, ethanol‐preserved, and paraffin‐embedded samples in DNA flow cytometryCytometry, 1989
- DNA flow cytometric values in bladder carcinoma biopsies obtained from fresh and paraffin‐embedded materialAPMIS, 1988
- Flow cytometric analysis of DNA in cells obtained from deparaffinized formalin‐fixed lymphoid tissuesCytometry, 1987
- Tumor ploidy as a major prognostic factor in advanced ovarian cancerCancer, 1987
- Comparison of DNA flow cytometry from fresh and paraffin embedded samples of non-Hodgkin's lymphoma.Journal of Clinical Pathology, 1985
- Application of DNA flow cytometry to paraffin‐embedded archival material for the study of aneuploidy and its clinical significanceCytometry, 1985
- The current status of fixation for electron microscopy: A reviewJournal of Microscopy, 1984
- Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry.Journal of Histochemistry & Cytochemistry, 1983
- Long‐term storage of samples for flow cytometric DNA analysisCytometry, 1983
- Limits of detection of nuclear DNA abnormalities by flow cytometric DNA analysis. Results obtained by a set of methods for sample‐storage, staining and internal standardizationCytometry, 1983