The Beta Chain of the GPIIb Molecule on Ruminant Leukocytes and Platelets Is Not Labelled by the Sulfo-NHS-Biotin Method
- 1 October 1995
- journal article
- Published by Wiley in Vox Sanguinis
- Vol. 69 (3) , 248-249
- https://doi.org/10.1111/j.1423-0410.1995.tb02603.x
Abstract
Glycoprotein (Gp)IIb/IIIa molecules have been immunoprecipitated from platelets and leukocytes of cattle, goat, horse, human, sheep and swine using specific monoclonal antibodies. The sulfo-NHS-biotin (sulfosuccinimidobiotin) method used to label the proteins has been found unsuitable for labelling the beta chain of the ruminant GPIIb/IIIa molecule. The beta chain was present on ruminant leukocytes and platelets when immunoprecipitates were silver stained.Keywords
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