Gas-Liquid Chromatography and Mass Spectrometry of Quinoxalines Derived from Various Homoglucans by Alkalineo-Phenylenediamine Method

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Abstract
Quinoxalines derived from various homoglucans by the alkaline OPD method were subjected to GLC and GC-MS analyses. The main quinoxalines derived from amylose and curdlan were identified as 2-hydroxymethyl- 3-(2′,3′-dihydroxypropyl)quinoxaline (M-l) and 2-(2′,3′,4′-trihydroxybutyl)quinoxaline (G-l), re-spectively, using a 2% OV-210 column after trimethylsilylation. Two quinoxalines M-l and G-l were formed from barley glucan and lichenan, and the approximate molar ratios of the 1,4- to 1,3- linkage in both glucans were estimated to be 1.6 and 2.2, respectively. From scleroglucan, G-l and(2′,3′-dihydroxy-4′-D-glucopyranosyl)quinoxaline were produced, reflecting a 1,3- and branched 1,6-linkage in the glucan. The ratios of the 1,4- to 1,3-linkage of barley glucan and lichenan, or the 1,6 to 1,3-linkage of scleroglucan estimated from the formed quinoxalines were in accordance with those obtained by conventional methylation analysis.

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