Identification of Canine Lymphocyte Populations by Immunofluorescence Surface Marker Analysis

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Abstract
A T-cell-specific rabbit anti-dog thymocyte antiserum was prepared and used in an indirect fluorescent antibody technique to identify T lymphocytes. The antiserum was rendered specific by absorptions with normal dog red blood cells, lyophilized serum, and bone marrow cells. By the indirect immunofluorescence assay, 73.6 ± 1.2 (SEM)% of the peripheral blood lymphocytes were identified as T lymphocytes. By direct immunofluorescence assay of surface membrane immunoglobulin-positive lymphocytes, 18.2 ± 0.8% of the peripheral blood lymphocytes were identified as B lymphocytes. The two assays used in conjunction can thus identify over 90% of the peripheral blood lymphocytes as either T or B lymphocytes, with the remaining 8.2 ± 1.0% as null cells.