A Study of the Mechanism of the Toluidine Blue Dye Test

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Abstract
Toluidine blue is commonly used by endoscopists as a screening test for squamous cell carcinoma. Little information is available on the mechanism of vital staining as it is not preserved by conventional fixation. By using a simple method to stabilize toluidine blue, the authors were able to study the action of the dye under the light and electron microscope. The findings are reported.