Molecular Cloning of Complementary DNA for Human Medullasin: An Inflammatory Serine Protease in Bone Marrow Cells
- 1 July 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 102 (1) , 13-16
- https://doi.org/10.1093/oxfordjournals.jbchem.a122024
Abstract
Medullasin, an inflammatory serine protease in bone marrow cells, modifies the functions of natural killer cells, monocytes, and granulocytes. We have cloned a medullasin cDNA from a human acute promyelocytic cell (ML3) cDNA library using oligonucleotide probes synthesized from the information of N-terminal amino acid sequence of natural medullasin. The cDNA contained a long open reading frame encoding 237 amino acid residues beginning from the second amino acid of natural medullasin. The deduced amino acid sequence of medullasin shows a typical serine protease structure, with 41% homology with pig elastase 1.This publication has 12 references indexed in Scilit:
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