Rifampidn inhibition of RNA synthesis by destabilisation of DNA-RNA polymerase-oligonucleotide-complexes
- 21 December 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (24) , 6889-6906
- https://doi.org/10.1093/nar/9.24.6889
Abstract
Although the antibiotic rifampicin inhibits the transcription of poly[d(A-T)] by E.coli RNA polymerase, a series of short oligonucleotides is produced. It is claimed that the overall inhibition of RNA synthesis by rifampicin is caused by a destabilising effect on the binding of the intermediate oligonucleotides to the active enzyme-DNA complex. Rifampicin itself can only interact specifically with RNA polymerase if the enzyme is free or in a binary complex with DNA. However, the enzyme is not susceptible in a ternary complex, even if the “RNA” is as short as a trinucleotide.Keywords
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