In vitroeffects of acephate on acetylcholinesterase activity in the brain and gills of rainbow trout,Salmo gairdneri

Abstract
The in vitro effects of acephate, a water‐soluble organophosphorus insecticide, on acetylcholinesterase (AChE) activity in rainbow trout were investigated over a range of 1,000 mg/L (5.5 mM) to 25,000 mg/L (136 mM) . In vitro concentrations required to produce 50% inhibition of AChE in the brain and gill were 12,600 mg/L and 14,100 mg/L respectively. Since such concentrations would not occur in natural waters following use of acephate to control terrestrial insects pest populations, fish mortalities would not be expected.

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