Alkyllysophospholipid ET‐18‐OCH3 acts as an activator of protein kinase C in HL‐60 cells
- 23 September 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 290 (1-2) , 231-234
- https://doi.org/10.1016/0014-5793(91)81267-c
Abstract
HL‐60 cells are very sensitive to the cytotoxic action of ether lipids. Several hypotheses have been proposed to explain this cytotoxicity. We investigated the influence of the alkylphospholipid ET‐18‐OCH3 on the activity of protein kinase C. HL‐60 cells were incubated with ET‐18‐OCH3 at a concentration of 20 μg/ml for 4 h. After the incubation the membrane fraction of the HL‐60 cells was isolated and the activity of protein kinase C was determined while it was still associated with the membrane, using the synthetic peptide substrate [Ser25]‐protein kinase C (19–31) as a protein kinase C specific substrate. The activity of the membrane‐bound protein kinase C was increased in HL‐60 cells treated with ET‐18‐OCH3 compared to untreated HL‐60 cells. The increase in protein kinase C activity was not a consequence of translocation and appeared to be additive to the effect of the phorbol ester 12‐myristate 13‐acetate. In contrast, solubilized protein kinase C from HL‐60 cells could be inhibited or stimulated in vitro by ET‐18‐OCH3, dependent on the mode of addition of ET‐18‐OCH3 and phospholipids.Keywords
This publication has 14 references indexed in Scilit:
- Activation of Protein Kinase C by Naturally Occurring Ether-linked DiglyceridesJournal of Biological Chemistry, 1989
- A novel method for measuring protein kinase C activity in A native membrane-associated stateBiochemical and Biophysical Research Communications, 1989
- Inhibition of Na,K-ATPase and sodium pump by anticancer ether lipids and protein kinase C inhibitors ET-18-OCH3 and BM 41.440Biochemical and Biophysical Research Communications, 1988
- Effects of antineoplastic ether lipids on model and biological membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1988
- Alkyl-linked diglycerides inhibit protein kinase C activation by diacylglycerolsBiochemical and Biophysical Research Communications, 1988
- A metabolite of an antineoplastic ether phospholipid may inhibit transmembrane signalling via protein kinase CLipids, 1987
- Accumulation of an alkyl lysophospholipid in tumor cell membranes affects membrane fluidity and tumor cell invasionLipids, 1987
- Translocation of protein kinase C in human leukemia cells susceptible or resistant to differentiation induced by phorbol 12-myristate 13-acetate.Proceedings of the National Academy of Sciences, 1986
- Staurosporine, a potent inhibitor of phospholipidCa++dependent protein kinaseBiochemical and Biophysical Research Communications, 1986
- Rapid purification of protein kinase C by high performance liquid chromatographyBiochemical and Biophysical Research Communications, 1986