Photometric Assay of Dopamine-β-Hydroxylase Activity in Human Blood
Open Access
- 1 September 1972
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 18 (9) , 980-983
- https://doi.org/10.1093/clinchem/18.9.980
Abstract
We describe a simple, rapid, and sensitive procedure for measuring dopamine-β-hydroxylase activity in human blood. The assay is based on the enzymatic conversion of tyramine to octopamine [1-(p-hydroxyphenyl)-2-aminoethanol], which is then oxidized to p-hydroxybenzaldehyde and determined photometrically. Activities are assayed on 2-20 µl of human serum or plasma obtained from fingertip blood samples. The method should prove useful in clinical studies, including the field of pediatrics.Keywords
This publication has 0 references indexed in Scilit: