ANTISERA FROM MICE AND RATS INOCULATED WITH SYNGENEIC ROUS-SARCOMA CELLS
- 1 January 1980
- journal article
- research article
- Vol. 50 (5) , 339-349
Abstract
CDF1, (BALB/c .times. DBA/2)F1, mice were repeatedly inoculated with transplantable mouse Rous sarcoma (SR-CDF1) [mouse brain tumor] cells which were half-killed by 60Co-irradiation beforehand to attain prolonged immunization. The sera obtained from these mice contained antibodies against the src and gag gene products of Rous sarcoma virus (RSV). Fischer rats inoculated with syngeneic rat Rous cells transformed by Bryan high titer strain of RSV (BH(-)-3Y1) produced antibodies against the gag gene products but not the src gene product of RSV. As a substrate for the protein kinase associated with the src gene product, mouse IgG was much less efficient than rabbit IgG when assayed in immunoprecipitates. Several other properties of the mouse antiserum were described.This publication has 18 references indexed in Scilit:
- Characterization of the avian sarcoma virus protein p60srcVirology, 1978
- Characterization of Rous sarcoma virus src gene products synthesized in vitroJournal of Virology, 1978
- Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoproteinCell, 1978
- Phosphorylation of chromatin- and ribosome-associated proteins in cells transformed by adenovirus, murine sarcoma virus, and methylcholanthreneJournal of Virology, 1978
- Protein kinase activity associated with the avian sarcoma virus src gene product.Proceedings of the National Academy of Sciences, 1978
- Identification of a transformation-specific protein induced by a rous sarcoma virusCell, 1978
- Transformation-defective mutants of Rous sarcoma virus with src gene deletions of varying lengthJournal of Virology, 1977
- In vitro translation yields a possible Rous sarcoma virus src gene product.Proceedings of the National Academy of Sciences, 1977
- Cell-free synthesis of two proteins unique to RNA of transforming virions of Rous sarcoma virus.Proceedings of the National Academy of Sciences, 1977
- Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.Journal of Biological Chemistry, 1977