Rapid determination and NMR assignments of antiparallel sheets and helices of a scorpion and a cobra toxin
- 31 August 1990
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 36 (3) , 227-230
- https://doi.org/10.1111/j.1399-3011.1990.tb00971.x
Abstract
An NMR method is described which should provide a rapid means for determining and assigning antiparallel sheets and helices in small proteins. It begins by locating apparent NOESY crosspeaks which suggest the presence of the secondary structure; this is followed by searches for MCD patterns (Englander and Wand (1987) Biochemistry 22, 5953) which are characteristic of these structures. As a result, only spin-systems of the amino acids within the secondary structure need to be defined. A triple-stranded, antiparallel sheet and a helix have been found and assigned for both .alpha.-cobratoxin and the scorpion toxin AaH III.Keywords
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