Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends
Top Cited Papers
Open Access
- 18 June 2007
- journal article
- review article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 35 (13) , 4223-4237
- https://doi.org/10.1093/nar/gkm389
Abstract
The possibility of performing fast and small-volume nucleic acid amplification and analysis on a single chip has attracted great interest. Devices based on this idea, referred to as micro total analysis, microfluidic analysis, or simply ‘Lab on a chip’ systems, have witnessed steady advances over the last several years. Here, we summarize recent research on chip substrates, surface treatments, PCR reaction volume and speed, architecture, approaches to eliminating cross-contamination and control and measurement of temperature and liquid flow. We also discuss product-detection methods, integration of functional components, biological samples used in PCR chips, potential applications and other practical issues related to implementation of lab-on-a-chip technologies.Keywords
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