Oxygen Consumption and Ouabain Binding Sites in Cystic Fibrosis Nasal Epithelium
- 1 December 1986
- journal article
- research article
- Published by Springer Nature in Pediatric Research
- Vol. 20 (12) , 1316-1320
- https://doi.org/10.1203/00006450-198612000-00026
Abstract
Ion transport by the epithelium lining the airways of patients with cystic fibrosis (CF) is characterized by a raised transepithelial PD and an increased amiloride sensitivity (1). These properties could arise from normal sodium transport across an epithelium with decreased cell chloride permeability and limited chloride secretion. Alternatively, a higher than normal rate of sodium absorption could contribute to these abnormalities. We investigated the latter possibility by measuring oxygen consumption and specific ouabain binding of CF and atopic polyp epithelia and normal turbinate epithelium. Tissue from CF patients consumed oxygen at a rate that was two to three times that of non-CF tissues and had 60% more ouabain binding sites than non-CF epithelium. These results are not consistent with an isolated defect in chloride permeability but support recent findings that the sodium conductance of the apical cell membrane and net sodium absorption by CF nasal epithelium are greater than those of non-CF nasal epithelium.This publication has 16 references indexed in Scilit:
- Ouabain binding in rectal gland ofSqualus acanthiasThe Journal of Membrane Biology, 1983
- Higher Bioelectric Potentials Due to Decreased Chloride Absorption in the Sweat Glands of Patients with Cystic FibrosisNew England Journal of Medicine, 1983
- Relative ion permeability of normal and cystic fibrosis nasal epithelium.Journal of Clinical Investigation, 1983
- Increased Bioelectric Potential Difference across Respiratory Epithelia in Cystic FibrosisNew England Journal of Medicine, 1981
- Thyroid gland function and pituitary TSH reserve in patients with cystic fibrosisThe Journal of Pediatrics, 1981
- Localization of Na pumps in the tracheal epithelium of the dog.The Journal of cell biology, 1979
- Mitrochondrial NADH dehydrogenase in cystic fibrosis.Proceedings of the National Academy of Sciences, 1979
- DIFFERENTIAL PATHOLOGY OF NASAL POLYPS IN CYSTIC-FIBROSIS AND ATOPY1979
- Localization of Na+-pump sites in frog skin.The Journal of cell biology, 1977
- CYSTIC-FIBROSISPublished by Elsevier ,1976