Regulation of protein synthesis in rabbit reticulocyte lysates
- 15 December 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 209 (2) , 162-164
- https://doi.org/10.1016/0014-5793(86)81103-6
Abstract
The specificity of the heme‐regulated protein kinase (HRI) was investigated further by utilizing the isolated 38000 Da subunit (α subunit) polypeptide of eIF‐2 as the substrate. For this purpose, the three subunit polypeptides of eIF‐2 (38000 Da, α, 50000 Da, β; and 52000 Da, γ) were resolved by reversed‐phase high performance liquid chromatography (HPLC). Results show that HRI is incapable of phosphorylating the 38000 Da subunit separated from the other two eIF‐2 polypeptides. Data suggest that the substrate'specificity of HRI is determined by the quaternary structure assumed by the α subunit in association with the other two subunits in the eIF‐2 holoprotein.Keywords
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