Cell Components in Proliferative Vitreoretinopathy: Immunofluorescent Double Staining of Cultured Cells from Proliferative Tissues

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Abstract
Intermediate filament proteins in cultured cells from epiretinal membranes of 5 cases of proliferative vitreoretinopathy and subretinal strands of 2 cases were immunohistologically characterized. Double staining technique after colcemid treatment revealed that either keratin-positive or vimentin-positive cells were found with different ratios depending on the cases. Both positive and both negative cells were also observed. Desmin and glial fibrillary acidic protein were totally negative.