Aminoacyl-tRNA Analogues; Synthesis, Purification and Properties of 3′-Anthraniloyl Oligoribonucleotides
- 1 April 1998
- journal article
- research article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 17 (4) , 815-829
- https://doi.org/10.1080/07328319808004677
Abstract
Reaction of isatoic anhydride with adenosine, adenosine 5′-phosphate, oligoribonucleotides or with the E. coli tRNAVal led to attachment of an anthraniloyl residue at 2′-or 3′-OH groups of 3′-terminal ribose residue. No protection of the S'-hydroxyl group or internal 2′-hydroxyl groups is required for this specific reaction. Anthraniloyl-tRNA which is an analogue of aminoacyl-tRNA forms a ternary complex with EF-Tu*GTP. The anthraniloyl-residue is used as a fluorescent reporter group to monitor interactions with proteins.Keywords
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