Biodehalogenation: Oxidative and reductive metabolism of 1,1,2‐trichloroethane by Pseudomonas Putida—Biogeneration of vinyl chloride

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Abstract
Resting cell suspensions of the camphor‐grown soil organism Pseudomonas putida PpG‐786, ATCC culture 29607, dehalogenate 1,1,2‐trichloroethane by two pathways under aerobic conditions. The dominant pathway (85%) is oxidative (kox, 4 × 10−19 L/org/sec) and leads in sequence to chloroacetic acid and glyoxylic acid. A competitive reductive pathway (15%) (kred, 0.7 × 10−19 L/org/sec) occurs simultaneously and yields vinyl chloride exclusively. Glucose‐grown cells are ineffective in these reactions, and carbon monoxide completely inhibits these transformations. These findings implicate cytochrome P‐450 cam as the responsible enzyme in vivo. Overall rates of conversion of 1,1,2‐trichloroethane by the organism and the isolated enzyme correspond to those obtained previously with ethylene dibromide.The results suggest that reductive microbial dehalogenation can occur in the environment even under aerobic conditions.