Acetate and bicarbonate metabolism in photosynthetic bacteria.

Publisher Website
Google Scholar
Abstract
The enzymes necessary for the synthesis of glutamic acid from acetate and bicarbonate through citric acid are present in extracts of Rhodospirillum rubrum. The addition of fluoroacetate to anaerobic resting cell suspensions which are capable of a light dependent synthesis of glutamate from acetate and bicarbonate inhibits glutamate synthesis with a concomitant increase in citric acid. This indicates that extracts of R. rubrum carry out glutamate synthesis from acetate and bicarbonate through citric acid. The fluoroacetate inhibition of a light dependent synthesis of glutamate from acetate and bicarbonate indirectly reflects the operation of an anaerobic tri-carboxylic acid cycle in resting cells for glutamate formation. The synthesis of glutamic acid from C1 and C2 precursors probably occurs in photosynthetic bacteria through an anaerobic tricarboxylic acid cycle.