THE CANINE MAJOR HISTOCOMPATIBILITY COMPLEX - POPULATION STUDY OF DLA-D ALLELES USING A PANEL OF HOMOZYGOUS TYPING CELLS

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  • 1 January 1983
    • journal article
    • research article
    • Vol. 21  (5) , 360-373
Abstract
The frequencies of 12 DLA-D alleles in a random canine population were determined in 1-way mixed lymphocyte cultures using a panel of homozygous typing cells established in this laboratory. The homozygous typing cells served as stimulators for responder lymphocytes obtained from 160 random dogs. The results were compared to those with lymphocytes from 75 dogs in the research laboratory. DLA-D allelic frequencies were stimated by maximum likelihood techniques. The use of a relative response (RR) .ltoreq. 5% as a definition of a typing response resulted in the recognition of a total allele frequency of 59% in dogs from the research laboratory. Three of the 12 DLA-D alleles were not detected. Typing responses of cells from random dogs to the 12 DLA-D alleles were determined using RR .ltoreq. 5%, .ltoreq. 10%, .ltoreq. 15% and .ltoreq. 20%. With RR of .ltoreq. 5%, .ltoreq. 10% and .ltoreq. 15%, the total allele frequencies recognized were 39%, 47% and 55%, respectively. Within each of these % RR ranges all but 1 of the DLA-D alleles were detected. With an RR .ltoreq. 20% of the total allele frequency recognized was 58% and all 12 alleles were detected. An RR of .ltoreq. 10% could be used to define a phenotypic DLA-D typing responses in the dog. The level of allelic frequencies detected in both the research and random canine populations indicates the need to identify additional DLA-D alleles through expanded family studies using mixed lymphocyte culture and homozygous cell typing.