STERIOD CONJUGATES IN HUMAN SEMINAL PLASMA

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Abstract
A technique for the measurement of 5 conjugated steroids (dehydroepiandro-sterone sulfate, pregnenolone sulfate, testosterone sulfate, dihydrotestosterone sulfate and testosterone glucuronide) in human seminal plasma is described. The steroid sulfates and testosterone glucuronide were measured by radioimmunoassay after solvolysis and enzyme hydrolysis, respectively. In all cases the recognized criteria of assay reliability were fulfilled. In a group of men with established fertility, dehydroepiandrosterone sulfate had the highest concentration and testosterone sulfate had the lowest (mean levels of 1.04 .mu.m/l and 1.52 nm/l, respectively). The levels of pregnenolone sulfate, dihydrotestosterone sulfate and testosterone glucuronide were 30.3, 4.37 and 38.8 nm/l, respectively. In all cases the levels of conjugated steroid exceeded the levels of the corresponding unconjugated compound.