Endoribonuclease IV A Poly(A)-Specific Ribonuclease from Chick Oviduct. 1. Purification of the Enzyme
Open Access
- 1 November 1976
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 70 (1) , 241-248
- https://doi.org/10.1111/j.1432-1033.1976.tb10975.x
Abstract
A new endoribonuclease, termed endoribonuclease IV, has been described. This enzyme has been isolated from chick oviducts and purified 15000-fold in a 25% yield nearly to homogeneity. The nuclease, which specifically degrades poly(A), forms oligonucleotides of an average chain length of 10. These fragments are terminated by 3′-hydroxyl and 5′-phosphate groups. The enzyme has a pH optimum at 8.7, requires Mn2+or Mg 2+ as a cofactor, and has a molecular weight of about 45000.Keywords
This publication has 19 references indexed in Scilit:
- The kinetics of pancreatic ribonuclease reaction with alkaline and acidic forms of poly AMolecular and Cellular Biochemistry, 1975
- Poly(A) polymerase in quail oviductBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- Association of an estradiol receptor with the DNA-dependent RNA polymerase I from immature quail oviductBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- Action of Bleomycin on DNA and RNAEuropean Journal of Biochemistry, 1972
- Localization of polyadenylic acid sequences in messenger ribonucleic acid of mammalian cellsBiochemistry, 1972
- Characteristics of the polyadenylic acid segment associated with messenger ribonucleic acid in mouse sarcoma 180 ascites cellsBiochemistry, 1972
- Micro estimation of RNA by the cupric ion catalyzed orcinol reactionAnalytical Biochemistry, 1969
- A simple assay for DNA endonucleasesAnalytical Biochemistry, 1965
- Sedimentation studies of the size and shape of DNAJournal of Molecular Biology, 1965
- Energetische Grundlagen der LebensvorgängePublished by Springer Nature ,1959