Inhibition of Cell Wall-Associated Peroxidase Isozymes with British Anti-Lewisite

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Abstract
Conditions were established for the use of British Anti-Lewisite (BAL) to inhibit preferentially the extracellular isoperoxidases in pea stems. After a 2 h incubation period with 9.09 x 104 M BAL, approximately half of the activity of cell wall-associated peroxidase was lost, while little inhibition of cytoplasmic enzyme was observed. Four isozymes from cell wall material were identified on starch gel e0lectrophoresis and stained with eugenol. Activity was tested with eugenol, ferulic and caffeic acids, and several redox dyes to probe the possible involvement of these isozymes in lignin biosynthesis. All isozymes had similar Km values with eugenol and caffeic acid as compared to benzidine, a standard redox dye.