SEPARATION AND DETERMINATION OF TOCOPHEROLS BY GAS CHROMATOGRAPHY

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Abstract
With the use of a highly sensitive gas chromatograph equipped with hydrogen ionization detector, separation and determination of [alpha]-, gamma- and [delta] -tocopherols were carried out. Following acetylation of the analogues, they were injected into the gas chromatograph in amounts of 5 [mu]l of 1[long dash]3% acetone solution after addition of squalene as the internal standard. Peak area was determined with a half value width method. A rectilinear relationship between the peak area and weight was obtained in [alpha] -, gamma- and [delta]-compounds within the range of 20[long dash]100 [mu]g. Recovery was 96.0-103.0%. Separation and determination of tocopherol analogues contained in the natural concentrate were easily carried out. Although colorimetry according to the National Formulary gave similar values of total vitamin, higher values of [alpha] -tocopherol were always obtained with colorimetry than those determined by gas chromatography, probably indicating a defect in the determination of non-[alpha] -compound in colorimetry.