3′,5′‐Cyclic Guanosine Monophosphate Activates Mitogen‐Activated Protein Kinase in Rat Pinealocytes
Open Access
- 1 August 1999
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 73 (2) , 598-604
- https://doi.org/10.1046/j.1471-4159.1999.0730598.x
Abstract
: The role of 3′,5′‐cyclic guanosine monophosphate (cGMP) in the activation of mitogen‐activated protein kinases (MAPKs) was investigated in rat pinealocytes. Treatment with dibutyryl cGMP (DBcGMP) dosedependently increased the phosphorylation of both p44 and p42 isoforms of MAPK. This effect of DBcGMP was abolished by PD98059 (a MAPK kinase inhibitor), H7 (a nonspecific protein kinase inhibitor), and KT5823 [a selective cGMP‐dependent protein kinase (PKG) inhibitor]. Elevation of cellular cGMP content by treatment with norepinephrine, zaprinast (a cGMP phosphodiesterase inhibitor), or nitroprusside was effective in activating MAPK. Natriuretic peptides that were effective in elevating cGMP levels in this tissue were also effective in activating MAPK. Our results indicate that, in this neuroendocrine tissue, the cGMP/PKG signaling pathway is an important mechanism used by hormones and neurotransmitters in activating MAPK.Keywords
This publication has 42 references indexed in Scilit:
- Orthovanadate Stimulates Cyclic Guanosine Monophosphate-Inhibited Cyclic Adenosine Monophosphate Phosphodiesterase Activity in Isolated Rat Fat Pads through Activation of Particulate Myelin Basic Protein Kinase by Protein Tyrosine KinaseEndocrinology, 1997
- Insulin and Insulin-Like Growth Factor-I Inhibit the L-Type Calcium Channel Current in Rat PinealocytesEndocrinology, 1997
- Atrial Natriuretic Peptide Inhibits Mitogen-activated Protein Kinase through the Clearance ReceptorPublished by Elsevier ,1996
- PD 098059 Is a Specific Inhibitor of the Activation of Mitogen-activated Protein Kinase Kinase in Vitro and in VivoJournal of Biological Chemistry, 1995
- Hormonal regulation of mitogen-activated protein kinase activity in bovine adrenocortical cells: cross-talk between phosphoinositides, adenosine 3',5'-monophosphate, and tyrosine kinase receptor pathwaysEndocrinology, 1995
- (Rp)-8-pCPT-cGMPS, a novel cGMP-dependent protein kinase inhibitorEuropean Journal of Pharmacology: Molecular Pharmacology, 1994
- Natriuretic peptides elevate cyclic 3',5'-guanosine monophosphate levels in cultured rat pinealocytes: evidence for guanylate cyclase-linked membrane receptorsMolecular and Cellular Endocrinology, 1994
- ERKs: A family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGFCell, 1991
- Multiple receptor regulation of cyclic nucleotides in rat pinealocytesProgress in Biophysics and Molecular Biology, 1989
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970