Enumeration, location, and characterization of endophytic bacteria within sugar beet roots

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Abstract
An increased bacterial population was found in the secondary root emergence zone tissue of sugar beet roots as compared with core and peripheral tissue of the same beet. The most common bacteria in sugar beet tissue were isolated, characterized, and used to immunize New Zealand white rabbits for antibody production. The bacteria-specific immunoglobulins from the rabbit sera were incubated with sugar beet tissue. These antibody-treated tissues were then incubated with ferritin-labeled goat antirabbit immunoglobulin to assist in identifying immunoglobulin-bound bacteria within tissue samples. This procedure was useful in distinguishing bacteria from artifacts and normal cell inclusions. The location of these antibody-bound bacteria within sugar beet root tissue was identified by using scanning electron microscopy.

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