Lymphocyte Populations: Separation by Discontinuous Density Gradient Centrifugation

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Abstract
A method for separation of human lymphocytes by discontinuous density gradient is described. The method was simple to carry out and utilized Ficoll-salt solutions which were easily standardized. There was good reproducibility with no toxicity to the cells. On applying this method to human thoracic duct lymphocytes, cells were separated into subpopulations which differed in morphology, basal unstimulated 3H-TdR incorporation and response to stimulation by purified phytohemagglutinin P and pokeweek mitogen.

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