The Specificity of Ribonuclease T2*
- 1 January 1967
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 61 (1) , 44-53
- https://doi.org/10.1093/oxfordjournals.jbchem.a128519
Abstract
RNase T2 [EG 2.7.7.17] has no absolute specificity as regards nucleotide bases, although it shows a preference for adenylic acid linkages in RNA. On exhaustive digestion with RNase T2, ribonucleic acids and homopolynucleotides are quantitatively digested to produce nucleoside 3'-phosphate. In the partial digestion of RNA with RNase T2, some phospho-diester bonds of Ap in RNA are preferentially split to produce the large fragments terminating with Ap in the very early stage of digestion, while few of the other nucleotide bonds are split. The susceptibility to RNase T2 is different depending on the molecular conformation of the substrates used.Keywords
This publication has 6 references indexed in Scilit:
- Purification and Properties of RNase T2*The Journal of Biochemistry, 1966
- Action of ribonuclease T2 on 2′, 3′-cyclic nucleotides and related compoundsArchives of Biochemistry and Biophysics, 1966
- Structure of a Ribonucleic AcidScience, 1965
- Adenylic acid arrangement in transfer RNA of yeastJournal of Molecular Biology, 1964
- Studies on the Specificity of Ribonuclease T2Journal of Biological Chemistry, 1963
- THE PREPARATION AND CHARACTERIZATION OF RIBONUCLEIC ACIDS FROM YEASTJournal of Biological Chemistry, 1955