Biodegradation of acyclic isoprenoids by Pseudomonas species

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Abstract
The ability of various pseudomonads to utilize acyclic isoprenoids as a sole C source was investigated. Tests for utilization of acyclic isoprenols such as citronellol aand geraniol were complicated by toxic effects of these alcohols, and most species tested were killed by exposure to citronellol or geraniol (0.1% vol/vol) in liquid culture. In the case of P. citronellolis, sensitivity to isoprenols is reduced by prior inducton of the isoprenoid degradative pathway via either growth on succinate in the presence of citronellol or growth on citronellic acid. For this species, citronellic acid proved to be the best isoprenoid growth substrate tested. Geraniol utilization as a taxonomic indicator for different subgroups of pseudomonads is discussed. Only a few of the species tested were able to utilize acyclic isoprenoids. Two species which utilize C10 acyclic isoprenoids, P. aeruginosa and P. mendocina, contained the inducible enzyme geranyl-CoA carboxylase, one of the unique enzymes in the isoprenol degradative pathway known to occur in P. citronellolis. Of the species which utilized geraniol, none showed definite growth on the homologous C15 and C20 isoprenols.