Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles
Top Cited Papers
- 4 February 2000
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 287 (5454) , 873-880
- https://doi.org/10.1126/science.287.5454.873
Abstract
Genome-wide transcript profiling was used to monitor signal transduction during yeast pheromone response. Genetic manipulations allowed analysis of changes in gene expression underlying pheromone signaling, cell cycle control, and polarized morphogenesis. A two-dimensional hierarchical clustered matrix, covering 383 of the most highly regulated genes, was constructed from 46 diverse experimental conditions. Diagnostic subsets of coexpressed genes reflected signaling activity, cross talk, and overlap of multiple mitogen-activated protein kinase (MAPK) pathways. Analysis of the profiles specified by two different MAPKs—Fus3p and Kss1p—revealed functional overlap of the filamentous growth and mating responses. Global transcript analysis reflects biological responses associated with the activation and perturbation of signal transduction pathways.Keywords
This publication has 77 references indexed in Scilit:
- The Transcriptional Program in the Response of Human Fibroblasts to SerumScience, 1999
- Drug target validation and identification of secondary drug target effects using DNA microarraysNature Medicine, 1998
- The Transcriptional Program of Sporulation in Budding YeastScience, 1998
- Membrane recruitment of the kinase cascade scaffold protein Ste5 by the Gβγ complex underlies activation of the yeast pheromone response pathwayGenes & Development, 1998
- Use of a cDNA microarray to analyse gene expression patterns in human cancerNature Genetics, 1996
- Identification of Yeast Rho1p GTPase as a Regulatory Subunit of 1,3-β-Glucan SynthaseScience, 1996
- New heterologous modules for classical or PCR‐based gene disruptions in Saccharomyces cerevisiaeYeast, 1994
- Identification of a gene necessary for cell cycle arrest by a negative growth factor of yeast: FAR1 is an inhibitor of a G1 cyclin, CLN2Cell, 1990
- Total synthesis of the lipopeptide a-mating factor ofSaccharomyces cerevisiaeBiochemical and Biophysical Research Communications, 1989
- New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sitesGene, 1988