Five additional genes in thepulC-O operon of the gram-negative bacteriumKlebslella oxytoca UNF5023 which are required for pullulanase secretion
- 1 July 1990
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 222 (2-3) , 176-184
- https://doi.org/10.1007/bf00633815
Abstract
DNA sequence analysis, Tnpho and Tntac-1, mutagenesis, deletion analysis, expression under bacteriophage T7 gene 10 promoter control, subcellular fractionation and complementation tests were used to study the function of DNA located in the centre of thepulC-O operon fromKlebsiella oxytoca strain UNF5023. The characterized region of the operon includes five genes (pulG,pulH,pulI,pulJ andpulK) coding for apparently integral inner membrane proteins which are required for pullulanase secretion. The results presented here and previously show that thepulC-O operon contains at least 11 pullulanase secretion genes.This publication has 21 references indexed in Scilit:
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