Chloramphenicol-Induced Loss of Mitochondrial DNA Polymerase Activity in HeLa Cells

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Abstract
HeLa [human cervical cancer] cells exposed to chloramphenicol for approximately one cell generation contained a mitochondria-associated DNA polymerase [EC 2.7.7.7] with a significantly lower specific activity than that of control cells. This observation was not due to the presence of inhibitors in mitochondrial DNA polymerase preparations of chloramphenicol-treated cell cultures. There was no accumulation of a typical mitochondrial DNA polymerase in the post-mitochondrial supernatant of drug-treated cells.